Browsing by Author "Afolabi OJ"

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    Incidence of urinary tract infections and antimicrobial susceptibility pattern among pregnant women in Akure, Nigeria
    (2019) Simon-Oke IA; Odeyemi O; Afolabi OJ
    Background Urinary tract infection (UTI) is the most common medical complications of pregnancy together with anemia and hypertension and it occurs approximately in 5–10% of all pregnancies. Objectives This study was carried out to evaluate the incidence and antimicrobial susceptibility pattern of isolated microbes among pregnant women attending ante natal clinics in Akure, Nigeria. Methods A total of 300 clean midstream urine samples were collected in a clean universal bottle. The urine samples were cultured, Microbact identification system was used to identify the presence of bacteria. Results Out of the 300 urine samples cultured, 183(61.0%) showed significant growth of urinary pathogens while 117(39.0%) showed lack of growth and insignificant growth of urinary pathogens. Escherichia coli 58(31.7%) was the dominant pathogen isolated, followed by Klebsiella pneumoniae 32(17.5%), Staphylococcus aureus 27(14.8%), and the least isolated were Citrobacter freudii and Providencia retgerri 2(1.1%) and 2(1.1%), respectively. Gram negative bacteria showed 77.6%, 67.9% and 61.2% susceptibility to Ciprofloxacin, Augmentin and Chloramphenicol, respectively. Resistance against Septrin, Chloramphenicol, Sparfloxacin, Amoxicillin, Augmentin, Gentamycin, Pefloxacin, Trivid and Streptomycin were observed in the range 23.1% to 70.1%. Gram positive uropathogens showed high resistance to Amoxicillin (68.4%) and high susceptibility to the other antibiotics in the range 65.8% to 89.5%. Conclusion There is need to treat pregnant women with UTIs with proven antibiotics that microbes are susceptible to in order to combat the evolution of resistant pathogens. Routine microbiological analysis and antibiotic sensitivity test of urine samples of pregnant women should be carried out so as to enhance the treatment and management of UTIs.
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    Prevalence and distribution of malaria, Pfcrt and Pfmdr 1 genes in patients attending FUT Health Centre, Akure, Nigeria
    (2018) Simon-Oke IA; Obimakinde ET; Afolabi OJ
    This research evaluated the distribution of malaria, Plasmodium falciparum chloroquine resistance transporter (Pfcrt) and Plasmodium falciparum multidrug resistant (Pfmdr1) mutant genes among patients attending FUTA Health Centre. The research was carried out between December 2015 and February 2016. One hundred and forty (140) patients coming for malaria test were examined out of which 74 (52.86%) were males and 66 (47.14%) were females. Malaria test was done by thick blood film microscopy and Polymerase Chain Reaction (PCR) technique. PCR technique was used to detect Pfcrt and Pfmdr1 mutant genes of P. falciparum. Information about sex, age, status and date of collection of each patient were collected. Pearson’s Chi-square test was used for analysis. Out of 140 blood samples that were tested, 100 (71%) were positive for malaria infection. The percentage distribution of malaria infection among male and female patients was 54 (72.97%) and 46 (69.70%) respectively with no significant difference (P=0.668). The prevalence of Pfcrt (95%) and Pfmdr1 (45%) showed significant difference (P=0.001). The research revealed high prevalence of Pfcrt and Pfmdr1 mutant genes of P. falciparum among patients which may be as a result of treatment of malaria with Chloroquine and other medication which the parasite have formed resistance against. It is therefore important to administer other malaria drugs apart from the drugs the parasite have formed resistance against.